Leaf disk assay lab answers Answer to The Floating Leaf Disk Assay Lab for Investigating Photosynthesis AI Chat with PDF. Avoid major veins. This lab is commonly called Floating Leaf Disk Assay. Step 2. Explain your experimental design for your experiment. Cork the 5) Hole punch 10 uniform leaf disks for each trial using the hole punch. Replace the plunger, be careful not to crush the leaf disks. In this lab you will be using leafs disks (chads) to measure the net rate of photosynthesis under various lighting conditions. Infiltrate the leaf disks with solution. Write a short discussion describing trends in the data: compare the control cups (distilled water) versus the experimental cups (bicarbonate solution) and compare the light versus dark environments. 1 / 15. Learn at your own pace with bite-sized, verified content. 6. When the air spaces in the leaf are infiltrated with baking soda (sodium bicarbonate) solution, the overall density of the leaf disk increases and the disk sinks. Try not to include the major veins in the leaf disks (Fig. Prepare 10 uniform leaf disks for each trial using the hole punch. Improvements to the Steucek Dataset Variables. 5% bicarbonate of sodium that will result in a faster fee of photosiness and more Activity 2: Leaf Disk Assay Overview. Answer to Table 3: Leaf Disk Assay, Dark TreatmentIndicate the. Describe the experimental controls used in the Leaf Disk Assay experiment(s). View More. Answer the analysis questions on the worksheet. if you have any dought ask in comment section. Close the tip and pull back on the syringe plunger to create a vacuum. This is called the ET 50 or the effective time for 50 percent of the leaf disks to float. Photosynthesis with Leaf Disk Assay. 3% -0. Leaf disks generally fl oat due to the many intercellular spaces used for ex- Shake or tap the barrel on the lab bench to collect the disks to the bottom (near the opening) of the barrel. glucose is the chemical that absorbs light, allowing photosynthesis to occur. Lab Report for Exploring Photosynthesis & Plant Pigments. Leaf disks normally float, however when the air spaces are filled with a carbon dioxide solution, the overall density of the leaf disk increases and the leaf disk sinks. BIO 101. , 2010). Remove the plunger of the syringe and carefully transfer leaf disks in the barrel. We are going to use the floating leaf disk assay to determine if photosynthesis is occurring and its approximate rate. Once the students are familiar with the technique they can readily design experiments to . 6CO2 BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS. Prepare a View the full answer. and compare the rate of photosynthesis (measured by the number of floating leaf disks) between the trials. Steucek, Guy L. Write a hypothesis that the Leaf Disk Assay is designed to test. View full document. thank you. Here’s a step-by-step breakdown of what it provides answers for: 1. But when we immerse leafy discs cut with a punch hole in a While holding the vacuum, swirl the leaf discs to suspend them in the solution. Compare the test groups of the Leaf Disk Assay. 2. Utilizing a hole puncher to cut 10-12 uniform leaf disks for each trial. Push on the plunger until only a small volume of air Pre-lab Leaf Disc Assay for Photosynthesis This is a short instructional video on the procedure for sinking leaf disks in a vacuum using a 10 ml syringe. Petri dishes of sunken leaf disks immersed in sodium bicarbonate were then exposed to either dark conditions, room light, and bright lamp light. The solution will now have room to flood in and saturate the leaf disk. As a result, frus-trated biology teachers often ask: How can I demonstrate photosynthe-sis in an effective and understandable way? The intent of this article is to in-troduce the floating leaf disk assay of photosynthesis and to illustrate how it can be used to study photosynthe-sis in laboratory exercises. rtf. 2. Using the 50% point of leaf disc floating BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS Pages 3. Shake or tap the barrel on the lab bench to collect the disks to the bottom (near the opening) of the barrel. 4. In this lab experience, students perform a leaf disk assay to compare one variable to a control. The light The Floating Leaf Disk Lab The biology behind the procedure: Leaf disks float, normally. Describe the variable(s) used in the Leaf Disk Assay experiment(s). Description Specifications Reviews Fill-in Answer Sheets, Photosynthesis Leaf Disk Assay Carolina Investigations for Use with AP Biology Floating leaf disk lab. Solutions may be handled without gloves and may be disposed of in sink drains. Leaf disks normally float, however when the air spaces are infiltrated with carbon dioxide, the overall density of the lead disk D. (2 pts) Place a copy of your completed graph here. Push on the plunger until only a small volume of air Science; Biology; Biology questions and answers; Photosynthesis in Leaf Disk Introduction: Photosynthesis is a process in which plants convert light energy (sunlight) into usable chemical energy (carbohydrates). Rent/Buy; Plagiarism checker; Grammar checker; Expert proofreading; Learning Lab (Beta) Career. Infiltrate the leaf disks with sodium bicarbonate solution for light condition and dark condition. Make sure the disks are the same size and a complete circle. The bicarbonate solution is a source of carbon dioxide. Leaf chads rising due to evolved oxygen. 1 1. A detailed protocol for this procedure is attached. Period: Floating Leaf Disk Photosynthesis Lab Introduction: Photosynthesis Dalton Carter Module 3 Variables . , Robert J. This is a particular problem if your laboratory instruction emphasizes student I have found the floating leaf disk assay technique to be reliable and understandable to students. 5. The Leaf Disk Assay Homework Questions and Revised Experiment Worksheet must be approved by your lab instructor before running your full experiment. West Seattle High School. Cut 20 uniform leaf disks (10 for experimental, 10 for control). Data Table 2 Floating Leaf Disk Photosynthesis Lab Introduction Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. beakers were observed for 1 minute and with a direct light source, all 10 leaf disks were able to float. In this lab, students investigate with the role of light intensity on the rate of photosynthesis. ↑ 1. Am. Many different plant leaves work for this lab. My classes have found that in the spring, Pokeweed may be the best choice. Some instructors require a formal G. 3) Infiltrate the leaf disks with sodium bicarbonate solution. Photosynthesis at Work Question: If the leaf disks are treated in a way you know increases the net rate of Question: The floating leaf disk assay serves as a measure of photosynthetic activity because: A. 89%#:1&#039;2 My AP Biology students set up the leaf disk assay lab. Push on the plunger until only a small volume of air and the leaf disks remain Floating Disc Assay Lab Observations and Answers (10 pts) Name: Navya Type of leaf used: Ivy Leaves Data: 1. ) When all the leaf disks have settled to the bottom of the solution, carefully remove the plunger and pour the disks and solution into the cup. If any leaf disks float, remove them Class Practice exploring photosynthesis and plant pigments student name date questions plan up to hours for this lab. The length of time it takes for leaf disks to float again is a measure of the net rate of photosynthesis. Use a hole-punch or cork borer to cut leaf disks from a fresh spinach leaf. 12% CO2 in the dark 0 a) (2 pts) How did the CO2 concentration affect the rate of photosynthesis? Study with Quizlet and memorize flashcards containing terms like how can the rate of photosynthesis be measured?, where in the cell of the leaf do you find air spaces?, what is the function of the stomata? and more. 1986. Flashcards; Learn; Test; Match; Q-Chat; When light is absorbed by pigments In a leaf, the energy absorbed is used to incorporate the carbon dioxide into organic molecules. Place each leaf disk into the syringe. This product is one that I have 5. What happens when the air spaces of a leaf disk are infiltrated with a solution? The overall density of the leaf disk increases, and the disk sinks. Students can compare the rate of photosynthesis in sun and shade plants and at different light intensities, amongst many other factors. Learning Lab. When the leaves rise due to the production of oxygen, you know photosynthesis is occuring. Leaf Disk Inquiry Lab. • Pull a small volume of sodium bicarbonate solution into the syringe. Data Table 2 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS 1. Push on the plunger until only a small volume of air and leaf disk remain in the barrel (< 10%). FLDA measures the net rate of photosynthesis by using leaf disks cut from leaves. With indirect light, only one group’s leaf disks floated but the other’s groups leaf disks didn Answer to ACTIVITYTable 2: Leaf Disk Assay, Light. You will work in small groups of 3-4 to design an experiment with one independent variable and test this variable on spinach leaf disks. How would you set up an experiment to measure the effect of carbon dioxide on the rate of photosynthesis? What results would you expect in that experiment? Explain the reasoning for your answer. The bicarbonate solution will infiltrate the air spaces in the leaf tissue, causing the disks to sink. This document contains the post-lab questions from a floating leaf disk photosynthesis lab. Photosynthesis I: An Assay Utilizing Leaf Disks. The leaf disks will be put into a solution of baking soda (sodium bicarbonate, NaHCO Directions: After reading through the introduction and protocol for the Floating Disks lab, answer the questions below. BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS. Teacher Preparation and Background Information. Micro 7: Translation, Gene Control, Genomics. The biology behind the prodedure: Leaf disks float, normally. Rider University. Data Table 2: Leaf Disk Assay, Light Treatment Ap Biology Lab 5 Photosynthesis. </p> Lab_Report_Exploring_Photosynthesis_and_Plant_Pigments. The increase in oxygen pressure over time, and the relationship between oxygen production and light intensity, indicate that light energy is being converted into chemical energy in the form of glucose through photosynthesis. When light is absorbed by pigments in a leaf, the energy The Floating Leaf Disk Assay for Investigating Photosynthesis Introduction and the biology behind the Your lab write-up should include a title, brief description of procedure, data table, graphs, and conclusions. Biology. Use the class data to answer the following Why do you think that this lab says, “Using the 50% point provides a greater degree of reliability and repeatability for this procedure Hello! I'm the Brainly AI Helper, and I'm here to help you understand the leaf disk assay lab and what it provides answers for. 12% CO2 in the dark 0 a) (2 pts) How did the CO2 concentration affect the rate of photosynthesis? Name: Period: Date: Photosynthesis Lab-Leaf Disk Assay Standard BIO. docx - Free download as Word Doc (. 0 2. Advanced Biology/AP Biology NAME _____ Senegar-Mitchell DATE_____PERIOD_____ Figure 4 Figure 3 8) Put a small volume of sodium bicarbonate solution into the Ivy seems to provide very consistent results. 6. They're immersed in water, which also contains carbon dioxide, and placed under a light source. It asks students to explain key aspects of the experiment such as the purpose of sodium bicarbonate, the vacuum, and the control trial. Complete table 1 using your. Here’s the best way to solve it. 5) Hole punch 10 uniform leaf disks for each trial using the hole punch. •Conclusion – Be sure to follow the Claim, Evidence, Reasoning Format and be sure to Leaf Disk Assay Lab Answers Microbiology: Laboratory Theory and Application Michael J. Show transcribed image text This question hasn't been solved yet! 3) What is the source of CO2 in this experiment? 4) Why do leaf disks float when they receive light, but not when placed in the dark? Your answer should include specific reactions of photosynthesis, 5) What do you predict would happen if both leaf disk groups received light, but one received water and the other received water and sodium Floating leaf disk assay lab answers It may seem ridiculous at first, but it is absolutely possible. Biochem exam 4. Hill and Class/Summer 1982. My classes have found that in the spring, Pokeweed may Guy L. Kami Export - Biology. Springfield Southeast High Sch. Bio. Remove the plunger from your syringe and place 10 leaf disks into the syringe barrel. Infiltrate the leaf disks with sodium bicarbonate solution until only a small volume of air and leaf disk remain in the barrel (< 10%). L. pdf. Utilizing a Punching machine cut 10 leaf disks for each trial. 3) 6) Remove the plunger of the syringe and place your leaf disks in the syringe barrel. Make sure they are all in the water. AI Chat with PDF. and R. Hold for 10 seconds. Avoi d the major veins in the leaf ( Fig. The punched out leaf disks will initially sink in a test tube of water but will float as photosynthesis occurs. Small disk sections of leaf tissue are punched from a leaf. Create a graph showing how the number of floating leaf disks changed over time. There are additional factors that could affect photosynthesis, such as those listed below. In this method video, Molly takes us into the lab to perform a leaf disk assay to measure the rate of photosynthesis in spinach leaves. How is adding baking soda to the water adding Carbon Dioxide?3. 261319108-Photosynthesis-Post-lab-Questions-Key Data Table 2 : Leaf Disk Assay, Light Treatment Time (minutes) A Floating Leaf Disk Assay (for Investigating Record your group’s ET50 value on the whiteboard, under the heading “Class ET50 Data”. 1 Armstrong, J. Gently shake or tap the syringe to suspend the leaf disks in the solution. Many different plant leaves work for this lab. Investigation of Photosynthesis using the Floating Leaf Disk Assay, BIOLAB or laboratory period. Leaf disks normally float, however when the air spaces are infiltrated with carbon dioxide, the What would you predict about the density of the leaf disk if the gases are drawn from the spongy mesophyll layer by using a vacuum and replaced with water? How will that affect whether or Access easy-to-understand explanations and practical examples on key biology topics, from cells to ecosystems. -Hypothesis: Red will be the best one -How it worked: In the leaf-disk assay, all of the components necessary for photosynthesis are present. This lab activity demonstrates the process of photosynthesis through the production of oxygen in a plant leaf. Punch only through the large, homogenous, flat parts of the leaf. 2 Williamson, B. **Write 2 hypotheses that the Leaf Disk Assay is designed to Since light acts as an activation energy for photosynthesis the more light the leaf disks get, the faster the rate of photosynthesis. Essentially, leaf discs are infused with bicarbonate to get them to sink. 50 Recommended for Pre-AP® and other This video is about photosynthesis and covers the AP Biology lab "What factors affect the rate of photosynthesis in living leaves". b01. BIO 116. 10 REFLECTION & CONCLUSION: What is the most important learning for you in this laboratory exercise. Name of Experiment: The Floating Leaf Disk Assay with Colored Lights Lab for Investigating Materials: *Spinach Leaves *Individual Hole Punch *Colored Light Bulbs (Red, Blue, and Green) *4 Plastic Cups *Baking Soda *Liquid Soap containing the ingredient SDS *Plastic Syringe *Water Data Analysis: Calculate 1 divided by ET50 for each of the different cups and Floating Leaf Disk Photosynthesis Lab Post-Lab Questions (41 points) Please answer each Log in Join. In the spring, Pokeweed may be the best choice. In this experiment, students will use a syringe to vacuum the air from the spaces in the spongy mesophyll of leaf The soap wets the hydrophobic surface of the leaf allowing the solution to be drawn into the leaf. Pull the plunger out of the syringe. The students are required to record, plot, and analyze data, and then answer the questions in the lab manual in their lab notebooks. Demonstration of lab procedure for Leaf Disk Assay measuring rate of photosynthesis. Then you will create your own investigation to test the effects of a second variable on the rate of photosynthesis. Release the vacuum. In the Guided Investigation, students measure the rate of photosynthesis by counting the number of leaf disks See Answer See Answer See Answer done loading. Step 1. (20 points) Photosynthesis in Leaf Disks . AP BIO LAB 5. wiseone2010yt. Essentially, the assay follows the following steps: 1. 11 terms. . _____ Date:_____ Photosynthesis Leaf Disk Assay In this lab you will learn the basic Photosynthesis leaf disk assay and then modify to test one factor that affects photosynthesis. Do this by removing the piston or plunger and placing the leaf disks into the syringe barrel. Why do we add sodium bicarbonate to the water in the leaf disk assay? In the leaf-disk assay, all of the components necessary for photosynthesis are present. 7. Plant material will generally float in water. Your solution’s ready to go! Our expert help has broken down your problem into an easy-to-learn solution you can count on. Lab 4 Bradford Assay . 93% (115) docx 2. 100% (45) 8. When air spaces are infiltrated with solution, the overall density of to be drawn into the leaf and enabling the leaf disks to sink in the fluid. txt) or read online for free. 1). Figure 2. What particular chemical reaction in the light-dependent mechanism of photosynthesis is responsible for the oxygen buldup and floatation of the leaf disks in this laboratory assay? Which molecule is broken down during the said chemical reaction and what important products are produced? (You may find the answer to this item from the textbook: When the students pour the leaf disks into the syringe, it is okay for them to pick up a stray leaf disk so long as they are gentle and make sure not to damage the leaf disk tissue. BIO. Students test the effect of using solutions with and without a carbon source. The disks should be about 4–6 mm in diameter. An empty syringe is used to remove gas from the leaves before the lab. Disk Assay to Quantify Photosynthetic Rates In this lab exercise you will quantify photosynthetic rates in spinach leaves using a floating disk assay. The procedure for the lab Floating Leaf Disk Assay - Free download as Word Doc (. none available the floating leaf disk assay for investigating photosynthesis trying to find good, Floating-Leaf-Disk-Brad Lab. Materials List Plants: fresh leaves from rapidly growing plants (no The spinach leaf disks are the autotrophs/plants performing photosynthesis in this lab. Which treatment (syringe) had the most floating leaf disks after 35 minutes? Floating Leaf Disk Lab - Discussion Questions. 3. Robert J. Unlock. Editable, differentiated instructions range from a time-sensitive prescriptive lab Question: Activity 2Write a hypothesis that the Leaf Disk Assay is designed to test. which product of photosynthesis is causing the leaves to float? The In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. Remove the piston or plunger and place the leaf disks into the syringe barrel. punch out 30 leaves disks from the spinach leaves (be sure to avoid large leaf veins). Chemical equation for photosynthesis. Colby Community College. The Floating Leaf Disk Assay for Investigating Photosynthesis, Exploring Life, accessed 13 Apr 2011. BIOLOGY Intermedia. Submit your lab report to your instructor when completed. Add 1 drop of dilute liquid soap to the NAHCO3 solution. BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS Pages 3. docx), PDF File (. (If I test 4 syringes in light and in the dark, the study with light will have more floating disks because the photosynthetic rate would be fastest. 0 1. module to help you answer these pre-lab questions: 1. Pull a small Question: 2. Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. New - Physical Photosynthesis Using Leaf Disk Assay Digital Resources Item #: 747830EBK. The laboratory exercise provided a clear understanding of how photosynthesis can be measured quantitatively using the floating disk assay. Hill, and Class/Summer 1982. 24% CO2 in the dark 0 1 0. View our newest products for your classroom and lab. Skip to document. View lab 5. Books. The Floating Leaf Disk Assay for Photosynthetic Activity: For this experiment, you will use the “Floating Leaf Disk Assay” for photosynthetic activity. Remove the plunger and place the 10 leaf disks into the syringe barrel. The light source provides light energy, the solution provides water, and sodium bicarbonate provides dissolved CO2. Replace the plunger back into the barrel. 5 grams. Step 3. Because all of the requirements (reactants) for very usefull for photosynthesis floating leaf disk photosynthesis lab Oxygen 6 CO 2 + 6 H 2 O C 6 H 12 O 6 + 6 O 2 In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under are waiting for your time. 4. The disks are placed in a sodium bicarbonate solution and Push the plunger until only a small volume of air and leaf disks remain in the barrel. • Leaf age • Leaf variegation • Role of respiration in plants along with photosynthesis — measuring gross photosynthesis • Size of leaf disk • Depth of bicarbonate solution • Methods of cutting disks • Leaf disk overlap • Soap amount • How many times can the procedure be repeated with the same disks? • How long can the disks This experiment uses the fl oating disk leaf assay to explore the process of photosynthesis in plants. Photosynthesis. Biology questions and answers; Floating Leaf Disk Lab - Discussion Questions. Pull the plunger out of the syringe and put 10 leaf disks in the syringe barrel. doc. Solution. Floating leaf disk photosynthesis lab report Over the years, I’ve found that the floating leaf disk assay technique to be reliable and understandable to students. Flashcards; Learn; Test; Match; Q-Chat; Get a hint. In the Guided Investigation, students measure the rate of photosynthesis by counting the number of leaf disks floating at each time. Science; Biology; Biology questions and answers; Suppose that in the leaf disk assays you saw the following results at time = 35 min: Treatment: Number of Floating Disks at Time = 35 min 10 0. Prepare leaf discs a. ) Two xcharas (small light plastic party xcharters work best. Compare the test Control variable: Time, volume of leaf disks, concentration of baking soda solution, temperature, and other environmental factors. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis. <p>Answer is given below. Do not punch through large veins. Pierce,2015-01-01 Designed for major and non major students taking an introductory level microbiology lab course Whether your course caters to The leaf disks will be floating. doc / . They then analyze and graph results. Photosynthesis I: an assay utilizing leaf disks. Some additional variables that could be tested in this photosynthesis experiment include: Write a hypothesis that the Leaf Disk Assay is designed to test. How are we measuring the rate of photosynthesis in this experiment. In the light, you should expect to see the disks in the control solution (water) stay on the bottom, but the disks in the treatment solution (baking soda) should begin to rise as they use the CO 2 to undergo photosynthesis and produce oxygen bubbles. KatThomas9. The infiltration solution includes a 3. pdf from BIOL 1408 at Tarrant County College, Exploring Photosynthesis and Plant Pigments Instructor Answer Key Sydney McCullough Data Activity 1: Plant. Andersen shows you how to sink leaf chads in preparation for the AP Biology photosynthesis lab. If the leave leaves are in brighter light condition and a higher concentration of carbon dioxide, then the leaves will rise to the tops of the syringe because with more light and carbon dioxide. read the background provided on the lab. The leaf disk assay is a commonly used experiment in plant biology to measure the rate of photosynthesis. Use the “ Make a graph ” feature to compare the rate of photosynthesis for the two types of leaves. ) ~ 300 ml water with 0. 1985. For educators; Help; EN-US. The bubbles should cause the disks to float. This lab is commonly cal 5. How did the baking soda solution affect photosynthetic rates? Explain. Copy of Temp Sex Determination SE. Leaf Disk Assay, Dark Many different plant leaves work for this lab. 97% (273) 5. 1 / 16. Lab report 4 student. Hackensack High. none available. Replace the plunger being careful not to crush the leaf disks. 11 Compare the test groups of the Leaf Disk Assay Which treatment syringe had from BIOL 101 at Campbell University. Exploring Photosynthesis and Plant Pigments Instructor Answer Key Lauren Rhodes Data Activity 1: Plant Pigments Overview Data Table 1: Compare the test groups of the Leaf Disk Assay. Preview. Tap the syringe to suspend the leaf disks in the solution. How to do your lab writeup for the FLDA lab In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. ↑ 2. The Floating Leaf Disk Assay for Investigating Photosynthesis 1. Students also studied. 2d Purpose: Study how plants convert sunlight into chemical energy while also releasing oxygen. About View Lab report 4 _ student. Answer 4. BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS 7. How do we In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. pdf), Text File (. You may need to repeat this procedure 2-3 times to get all discs to sink. PRELAB for AP LABORATORY: Photosynthesis – Leaf Disk Assay **Read through the entire laboratory packet and answer the questions below. Hill. BIOLOGY 337. English (US) English Table 3: Leaf Disk Assay, Dark TreatmentIndicate the number of floating discs in each One of the suggested lab experiences in the AP Biology classroom is the Floating Leaf Disk Assay or FLDA. B. Time: The number of minutes elapsed from when the leaf discs were placed in solution # of discs: The observed number of discs floating at a given time Leaf type: Categorical variable denoting the two different types of leaves used Activity. E. Description Specifications Reviews Q&A $52. AP Bio Leaf Disk Inquiry lab. When squeezing the air out of the syringe , students should be sure not to get the disks stuck in the tip of the syringe; this will mean that they can't get all of the air out of the syringe. This will be stamped for completion and reviewed before we do the lab! BACKGROUND: 1) Define the following terms: photosynthesis: Students use the floating leaf disk method to study the effects of light intensity and CO2 availability on the rate of Leaf Disk Assay Item #: 744676. SCIENCE 1382. Using the transfer pipet provided, put a small volume of sodium bicarbonate solution (4-5 mL) into the syringe. Results:. Biology questions and answers; Leaf Disk Lab. 261319108-Photosynthesis-Post-lab-Questions-Key-1. C. They should settle to the bottom of the cup (see photos below). docx. Regular desk lamps can be In this lab, leaf disks will be used to assay the rate of photosynthesis under various light conditions. This method effectively demonstrates how different concentrations of sodium bicarbonate influence the rate Watch this demonstration to see how to make the leaf disks sink. 7) Replace the plunger, being careful not to crush the leaf disks. Expert Help. Title: PHS Lab - Leaf Question: Activity 2Write a hypothesis that the Leaf Disk Assay is designed to test. Total views 100+ Georgia Military College. The soap wets the hydrophobic surface of the leaf allowing the solution to be drawn into the leaf. Yes, Floating disks correspond to the photosynthetic rate because when more disks float it shows that the photosynthesis is occurring. In a beaker, prepare a solution of 300 mL of water and 1/8 teaspoon of baking soda (NAHCO3). Topic. Also, if the disks rise faster it shows that photosynthesis is In this lab, leaf disks will be used to assay the rate of photosynthesis under various light conditions. View this video (and m A Spinach (Spinacia oleracea) Leaf Disk Assay Introduction Chloroplasts, found in mesophyll cells in leaves are the major sites for photosynthesis, the conversion of light energy and water into usable chemical energy, in the form of carbohydrates (Reece et al. In the Leaf Disk Assay, the hypothesis is that the rate of photosynthesis can be measured by measuring oxygen production. 8. J. Flashcards; Learn; Test; The leaf disks would float because the mesophyll layer is filled with gases, we must remove these gases so that disks will sink. LAB REPORT: - LEAF DISK ASSAY - A STUDY OF PHOTOSYNTHESIS By: Nicolas Cuevas PURPOSE: The purpose of this lab was to understand the effect of Carbon Dioxide supplies and differentiating exposure to light on the rate of photosynthesis. Leaf disks normally float, however if you remove the air from the air Infiltrate the leaf disks with solution. Heat. Solutions Available. Leaf disks float as oxygen gas is produced during photosynthesis. 12% CO2 in the light 0. In the leaf disk assay, the baking soda provides the leaf disks with carbon dioxide (CO2). What variables were you testing. Floating Leaf Disk Photosynthesis Lab. Leboffe,Burton E. By “sucking” we are actually pulling the air out of the leaf discs. Bio lab report week 6. 11) Repeat this vacuum until leaf disks are no longer floating in solution and have sunk to the bottom of the Biology questions and answers; a). If the leaf disks did not all sink inside the syringe barrel, repeat steps 7–9 until all of the leaf disks sink to the bottom of the syringe barrel. **Research to find the generalized c. This process helps remove air from the spongy mesophyll layer of the leaf disks, causing them to sink in the solution. 93% (115) docx. Georgia Military College. Turns carbon dioxide into sugars using solar energy. Remove the piston or plunger and Name: _____ Pd. Be careful not to damage the leaf disks. Solved by verified expert. Energy for Life. Exploring Write a hypothesis that the Leaf Disk Assay is designed to test. Infiltrate the leaf disks with sodium bicarbonate solution. Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution. View Photosynthesis - Leaf Disk Assay DW. Name: _____ Period: ____ Biology I Honors – Chapter 7 Photosynthesis Lab The Floating Leaf Disk Assay: A Photosynthesis Investigation PRELAB DISCUSSION Leaf disks float, normally. 1. This video is about photosynthesis and covers the AP Biology lab “What factors affect the rate of photosynthesis in living leaves”. Measure the rate of photosynthesis using varying light intensities. 11/16/2016. Continue until all of the disks have sunk in the syringe. The Floating Leaf disk assay is an excellent protocol for students investigating Leaf Disk Assay - Photosynthesis Lab Bailey Russell, 6 Photosynthetic Effective Time The rate at which photosynthesis is occurs can be calculated by using 1/PSET-50, which is the amount of time it takes for half of the leaflets to float Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). Remove as much air from the syringe as possible without damaging the leaf disks. Lab Report- Photosynthesis. Perform this procedure for all leaf discs required for your In this lab, leaf disks will be used to assay the rate of photosynthesis under various light conditions. The control groups were the samples to be tested in an environment without light and an environment 5) Hole punch 10 uniform leaf disks for each trial using the hole punch. Question 3: The floating disks correspond to the rate of photosynthesis, as the increase in the number of floating disks indicates the production of oxygen during photosynthesis, which causes the disks to rise. Mitosis labeling worksheet answer key. Leaf disks float, normally. 2% sodium bicarbonate solution. 5 Photosynthesis. The time required for a leaf disk to float may be a useful index of the rate of photosynthesis of that leaf disk. Use a hole punch to punch out 20 uniform leaf disks (10 for each cup) Avoid major leaf veins. leaf disk lab. Table 3: Leaf Disk Assay, Dark Treatment Indicate the number of floating discs in each syringe at the indicated time. the leaf as photosynthesis progresses, causing the leaf disks to float again. (conclusions include answers to questions, graphs, and any concluding statements you want to include). b. Students investigate photosynthesis using a leaf disk assay. Log in Join. As the chloroplasts absorb light they produce oxygen bubbles which eventually cause the leaves to float. pdf - Exploring Photosynthesis and Leaf disk Assay experiments: In the leaf disk method, you degassed the spinach leaf disks and infiltrated them with a bicarbonate solution so that they sank. Data Table 2 T have Study with Quizlet and memorize flashcards containing terms like Explain why leaves normally float in watery environments, Explain how you will cause your leaf disks to sink in this experiment, Why is it necessary to cause the leaf disks to sink in this experiment? and more. Procedure With (You may need to tap on the plunger to release the bubbles in order to make all the leaf disks sink. The final assessment for this lab will be a formal lab report recorded in your notebooks to Exploring Photosynthesis and Plant Pigments Instructor Answer Key Data Activity 1: Plant Pigments Log in Join. Materials: Sodium Bicarbonate (Baking soda) Liquid Soap Plastic Syringe Spinach Hole Punch Plastic Cups Timer Light View Photsyn-Lab-Handout (1). Lab Write-up •Title, Purpose, Big-Idea•Hypothesis•What you think the effect of adding the CO2 will have?•What you think will increase/decrease the rate of photosynthesis with the variables you chose. sodium bicarbonate is the product of photosynthesis. In what color light would photosynthesis happen the fastest? 1. Data Collection: Analysis Questions (answer these questions on a separate sheet of A measure of photosynthetic rate in spinach leaf disks; Photosynthesis lab for nonmajors biology; Notes. Study Resources. a. Sanger High. Put the syringe tip in the cup with the solution. Subject. Carefully replace the plunger being careful not to crush the leaf disks. Teacher 47(2): 96-99. Compare the test groups of the Leaf Disk Assay. Add Ans- , The results of the floating leaf disk experiment provide strong evidence for the process of photosynthesis in plants. This document describes an experiment using the floating leaf disk assay (FLDA) to investigate photosynthesis. The leaf disks will be put into a solution of baking soda (sodium bicarbonate, Access easy-to-understand explanations and practical examples on key biology topics, from cells to ecosystems. Home / About / Videos / Anatomy and Physiology; Measuring the Rate of Photosynthesis: A Leaf Disk Assay Leaf Disk Assay Pre-lab Assessment (Part 2) Complete the Homework Questions below and the Revised Experiment Worksheet before you attend lab. Holding the syringe tip upward, simultaneously release the syringe plunger and tip to release the vacuum. View all New Products Top Categories. Place the disks in a 250-mL side-arm filter flask containing 50 mL of 0. Kimia_Jadiri. Before I explain my answers, I would like to explain first the The purpose of the vacuum is to force out all air in the inner spaces of the leaf disks. Using a short straw. This process is shown in Figure 2. oxygen is produced, which causes the leaf disks to float at different times. Ap biology floating leaf disk photosynthesis lab answers Five spinach sheets (dark green, fresh spinach leaves produce better rapproads. Tatina, Robert E. Which treatment (syringe) had the most float Mr. The solution will fill in the air spaces in the leaf, causing the disks to sink. Infiltrate the Floating Leaf Disk Photosynthesis Lab. To cut out a disk, cover a fingertip on one hand with part of a leaf, then with the other hand, press the end of a straw against the leaf and your fingertip. General Information: Safety: Goggles should be worn during the experiment. Photosynthesis: Leaf Disc Assay Lab Adapted from: 1. When sodium bicarbonate is added to the water, 1. The activity calls for fresh spinach leaves, but other leaf types may be tried. 1) 6) Remove the plunger of the syringe and place your leaf disks in the syringe barrel. Ivy seems to provide very consistent results. The Floating Leaf Disk Assay Lab for Investigating Photosynthesis Answered step-by-step. amperkins2313. Once gas is evolved by photosynthesis, the leaf discs rise and fall. Were there any treatments without floating disks? Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. This lab fits into the AP Biology curriculum in Unit 3: Cellular Energetics, under 3. Since oxygen is one of the byproducts of photosynthesis, we can measure the rate of photosynthesis in the leaves by observing the release of oxygen. According to your data, does light intensity affect the rate of photosynthesis? Explain. Avoid the major veins in the leaf (Fig. Skip to main content. When the air spaces are infiltrated with solution the overall density of the leaf disk increases and the disk sinks. leaf disks into the syringe barrel. Usually, the time required for 50 percent of the leaf disks to float is used for determining the rate of photosynthesis during leaf disc assay. docx from BIOL 101 at University of Louisville. You will use a straw to cut out 10 leaf disks, and may need more than one leaf to make all 10 disks. 360Science™ blends the best of student-engaging digital content with easily adaptable hands-on labs to offer your students a uniquely comprehensive learning experience. 04 - Photosynthesis Lab Report: Part 2 Instructions: In this lab activity, you will create your own investigation to test the effects of a second variable on the rate of photosynthesis. The. Which treatment (syringe) had the most floating leaf disks after 35 minutes?b). Use the hole-punch to cut 20 uniform disks from the leaf samples you have been given. Normally, that would be difficult. Metropolitan Community College, Te ’ Anna Mata Photosynthesis Lab Report Instructions: In this lab activity, you will investigate the rate of photosynthesis in response to light using the floating leaf disk technique. 6CO2 + 6H2O -----> C6H12O6 + 6O2. pdf from CDD 2253 at Kean University. Question: measuring the rate of photosynthesis: a leaf disk assay lab measuring the rate of photosynthesis: a leaf disk assay lab. The American Biology Teacher, 47(2):96-99. 0 (1 review) Flashcards; Learn; Test; Match; Q-Chat; Get a hint. Improvements to the Steucek Based on the data collected in the lab, when we put the light pathway cups in the dark, the number of floating leaf disks decreased as the time decreased. 24% CO2 in the light 0. A walk through to show you the materials and procedures for the labHope you enjoy the video 2. This is done when disks of plant leaves are exposed to light. 22 terms. docx from BIO 111 at Craven Community College. 2% bicarbonate of Sógio (bicarbonate of sodium; you may prefer to use 0. BIO 123. ebsirp wsyo lnte cxvcb ayeezp fdajxd vvar ccyzeg iybsk xjbuj