Leaf disk assay lab answers. Total views 100+ Georgia Military College.

Leaf disk assay lab answers Photosynthesis requires water, carbon Photosynthesis Lab Report Instructions: In this lab activity, you will investigate the rate of photosynthesis in response to light using the floating leaf disk technique. The final assessment for this lab will be a formal lab report recorded in your notebooks to Unhealthy or very veiny leaves may also be an issue. Floating Leaf Disk Photosynthesis Lab Introduction: Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. What variables were you testing. com/labtools/Leafdisk. 4) Figure 1 Figure 2 This is a particular problem if your laboratory instruction emphasizes student-generated questions. 9. The light source provides light energy, the solution provides water, and sodium bicarbonate provides dissolved CO2. Aug 22, 2018 · The students are required to record, plot, and analyze data, and then answer the questions in the lab manual in their lab notebooks. Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. This document contains the post-lab questions from a floating leaf disk photosynthesis lab. Try not to include the major veins in the leaf disks (Fig. Data Table 2 Answer to ACTIVITYTable 2: Leaf Disk Assay, Light. The oxygen bubbles that are produced are Mar 18, 2020 · Watch this demonstration to see how to make the leaf disks sink. Here’s the best way to solve it. Once the students The spinach leaf disks are the autotrophs/plants performing photosynthesis in this lab. The rate of photosynthesis in the disks of the leaf tissue is deter-mined by noting the time required for submerged leaf disks to float. **Were there Nov 3, 2024 · Cut 10 leaf disks from the leaf using the hole punch. Tap the syringe to suspend the leaf disks in the solution. 360Science™ blends the best of student-engaging digital content with easily adaptable hands-on labs to offer your students a uniquely comprehensive learning experience. Carbon dioxide for photosynthesis sourced from bicarbonate solution. Learn at your own pace with bite-sized, verified content. Question: Floating Leaf Disk Lab - Discussion Questions. BIOS 1010 General Biology 3. The bubbles should cause the disks to float. Lab Report- Photosynthesis. Remove the piston or plunger and place the leaf disks into the syringe barrel. The laboratory exercise provided a clear understanding of how photosynthesis can be measured quantitatively using the floating disk assay. 12%) B (0. If the leaf disks did not all sink inside the syringe barrel, repeat steps 7–9 until all of the leaf disks sink to the bottom of the syringe barrel. . How did the baking soda solution affect photosynthetic rates? Explain. Exploring Photosynthesis and Plant Pigments - Exploring Pages 4. In the light, you should expect to see the disks in the control solution (water) stay on the bottom, but the disks in the treatment solution (baking soda) should begin to rise as they use the CO 2 to undergo photosynthesis and produce oxygen bubbles. 5 terms. We are going to use the floating leaf disk assay to determine if photosynthesis is occurring and its approximate rate. Explain your experimental design for your experiment. Also, if the disks rise faster it shows that photosynthesis is Apr 16, 2021 · In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. Preview. Photosynthesis in Leaf Disks . The bicarbonate solution is a source of carbon dioxide. BIO. Sarah_Henriquz505. When light is absorbed by pigments In a leaf, the energy absorbed is used to incorporate the carbon dioxide into Oct 17, 2024 · 3. The process of photosynthesis can Mar 6, 2021 · Floating leaf disk assay lab answers It may seem ridiculous at first, but it is absolutely possible. Pull the plunger out of the syringe and put 10 leaf disks in the syringe Aug 16, 2021 · Floating Leaf Disk Assay (for Investigating Photosynthesis) by Brad Williamson. This lab activity demonstrates the process of photosynthesis through the production of oxygen in a plant leaf. Infiltrate the leaf disks with sodium bicarbonate solution. Use the “ Make a graph ” feature to compare the rate of photosynthesis for the two types of leaves. Oxygen produced displaces liquid, causing disks to float. L. Petri dishes of sunken leaf disks immersed in sodium bicarbonate were then exposed to either dark conditions, room light, and bright lamp light. Leaf disks normally float, however when the air spaces are Leaf disks normally float, however when the air spaces are infiltrated with carbon dioxide, the overall density of the lead disk increases and the leaf disk sinks. In this lab, factors of photosynthesis were tested via floating disk assay. Total views 100+ Georgia Military College. Unlock. Steucek, Guy L. Then you will create your own investigation to test the effects of a second variable on the rate of photosynthesis. 2. The study of light's effect on 3. The Floating Leaf disk assay is an excellent protocol for students investigating photosynthesis. Push on the plunger until only a small volume of air and leaf disk remain in the barrel (&lt; 10% of barrel. Generally speaking, a leaf floats (due to the many intercellular spaces used for exchange of gases). 12) Pour the leaf disks and their solution into the appropriately labeled cup. Teacher 47(2): 96-99. Leaf Disk Pre-lab. Time: The number of minutes elapsed from when the leaf discs were placed in solution # of discs: The observed number of discs floating at a given time Leaf type: Categorical variable denoting the two different types of leaves used Activity. Because all of the requirements (reactants) for photosynthesis are present, photosynthesis is able to occur and produce both oxygen gas and glucose. As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy--causing the disks to rise. Leaf chads rising due to evolved oxygen. , 2010). They're immersed in water, which also contains carbon dioxide, and placed under a light source. Oct 21, 2018 · In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. 24%) Average of A and B A (0. 12% CO2 in the light 0. The larger the Nov 26, 2023 · Final answer: The leaf disk assay lab provides answers related to the effectiveness of different test solutions on the rate of photosynthesis. Do this by removing the piston or plunger and placing the leaf disks into the syringe barrel. Shake or tap the barrel on the lab bench to collect the disks to the bottom (near the opening) of the barrel. Students can compare the rate of photosynthesis in sun and shade plants and at different light intensities, amongst many other factors. General Information: Safety: Goggles should be worn during the experiment. Note that some of the O 2 produced by photosynthesis is used for cellular respiration. This lab is commonly called Floating Leaf Disk Assay. Utilizing a hole puncher to cut 10-12 uniform leaf disks for each trial. 4. In the chart above, draw arrows to indicate how the products of cellular respiration can be used for photosynthesis and the Vacuum infiltration removes intracellular air from leaf disks. 12% CO2 in the dark In the leaf-disk assay, all of the components necessary for photosynthesis are present. Leaf disks generally fl oat due to the many intercellular spaces used for ex-change of gases. 2d Purpose: Study how plants convert sunlight into chemical energy while also releasing oxygen. C. A Spinach (Spinacia oleracea) Leaf Disk Assay Introduction Chloroplasts, found in mesophyll cells in leaves are the major sites for photosynthesis, the conversion of light energy and water into usable chemical energy, in the form of carbohydrates (Reece et al. Am. _____ Date:_____ Photosynthesis Leaf Disk Assay In this lab you will learn the basic Photosynthesis leaf disk assay and then modify to test one factor that affects photosynthesis. As photosynthesis proceeds, oxygen accumulates in the air spaces of the spongy mesophyll and the leaf becomes buoyant and floats. But when we immerse leafy discs cut with a punch hole in a module to help you answer these pre-lab questions: 1. Make sure the disks are the same size and a complete circle. Which treatment (syringe) had the most float 2. 04 - Photosynthesis Lab Report: Part 2 Instructions: In this lab activity, you will create your own investigation to test the effects of a second variable on the rate of photosynthesis. This lab fits into the AP Biology curriculum in Unit 3: Cellular Energetics, under 3. The soap breaks down the molecules on the leaf’s surface (because the leaf repels the solution) to allow the bicarbonate solution to penetrate the cells. Once the students are familiar with the technique they can readily design experiments to answer their own questions about Students investigate photosynthesis using a leaf disk assay. Explanation: The leaf disk assay lab is a method used in biology to measure the rate of photosynthesis. Prepare a View the full answer. Essentially, leaf discs are infused with bicarbonate to get them to sink. See Answer See Answer See Answer done loading. The floating leaf disk assay serves as a measure of photosynthetic activity because: A. Results:. The punched out leaf disks will initially sink in a test tube of water but will float as photosynthesis occurs. Nov 16, 2016 · BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS Pages 3. b. Submit your lab report to your instructor when completed. Jul 21, 2022 · This experiment uses the fl oating disk leaf assay to explore the process of photosynthesis in plants. • Remove the piston or plunger and place the leaf disks into the syringe barrel. pdf from BIOL 1408 at Tarrant County College, Fort Worth. They then analyze and graph results. Turns carbon dioxide into sugars using solar energy Lab 8 & 9. In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. Lab Write-up •Title, Purpose, Big-Idea•Hypothesis•What you think the effect of adding the CO2 will have?•What you think will increase/decrease the rate of photosynthesis with the variables you chose. Which treatment (syringe) had the most floating leaf disks after 35 minutes? 10. Remove the plunger of the syringe and carefully transfer leaf disks in the barrel. Describe the experimental controls used in the Leaf Disk Assay experiment(s). Leaf Disk Assay Item #: 744676. The light source was originally described in Steucek, Guy L. What happens because cellular respiration is also taking place? The oxygen consumed because of photosynthesis is an indirect measurement of the net rate of photosynthesis. Prepare leaf discs a. Mar 17, 2022 · Spinach Leaf Disks Amelia Robinson-Brown Heathwood Hall Episcopal School, Columbia, SC 29205 The purpose of this experiment was to determine the effect of carbon dioxide concentration on the rate of photosynthesis in spinach leaves. docx), PDF File (. Flashcards; Learn; Test; Match; Q-Chat; Get a hint. Regular desk lamps can be Browse the latest lab supplies and equipment for all your science lab essentials. o Part A: Aim – to explore a method that uses leaf discs to measure rate of photosynthesis duced accumulates in the leaf disk imparting buoyancy; the leaf disk floats. Normally, that would be difficult. elbiology. 12%) Average of A and B 0 5 10 15 20 25 30 35 40 45 50 55 60 3. Exploring Photosynthesis and Plant Pigments Instructor Answer Key Lauren Rhodes Data Activity 1: Plant Pigments Overview Data Table 1: Leaf disk Assay experiments: Before I explain my answers, I would like to explain first the chemical equation for photosynthesis. The lab Oct 14, 2020 · View lab 5. 3) What is the source of CO2 in this experiment? 4) Why do leaf disks float when they receive light, but not when placed in the dark? Your answer should include specific reactions of photosynthesis, 5) What do you predict would happen if both leaf disk groups received light, but one received water and the other received water and sodium Dec 9, 2021 · Teacher Notes and Laboratory Preparation GTAC Preparing for student leaf disc experiments 1 Using leaf discs to investigate photosynthesis – advice for teachers and laboratory technicians There are three practical activities associated with this resource. J. Step 1. Push on the plunger until only a small volume of View Lab report 4 _ student. You will work in small groups of 3-4 to design an experiment with one independent variable and test this variable on spinach leaf disks. Explain why leaves normally float in watery environments. Biology questions and answers; QUESTION 8 The floating leaf disk assay serves as a measure o photosynthetic activity because: (2 pt] O glucose is the chemical that absorbs light, allowing photosynthesis to occur. 3. When light is absorbed by pigments in a leaf, the energy absorbed is used to incorporate the carbon dioxide into organic molecules in a process called carbon fixation. When the leaves rise due to the production of oxygen, you know photosynthesis is occuring. Write a hypothesis that the Leaf Disk Assay is designed to test. Prepare 10 uniform leaf disks for each trial using the hole punch. Plant This is a particular problem if your laboratory instruction emphasizes student-generated questions. Title: Include the name of the lab (be descriptive) and your name. Apr 14, 2024 · in the leaf (Fig. Students investigate photosynthesis using a leaf disk assay. ) 6. Which treatment (syringe) had the most float Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). 4. Essentially, the assay follows the following steps: 1. The pandemic has forced schools, like ours, to do almost Unformatted text preview: B (0. This will be stamped for completion and reviewed before we do the lab! BACKGROUND: 1) Define the following terms: photosynthesis: chloroplast: This is a particular problem if your laboratory instruction emphasizes student-generated questions. Infiltrate leaf disks with vacuum to remove air. Measure the rate of photosynthesis using varying light intensities. docx from BIOL 101 at University of Louisville. Compare the test groups of the Leaf Disk Assay. Materials List Plants: fresh leaves from Nov 27, 2021 · Floating Leaf Disk Photosynthesis Lab Post-Lab Questions (41 points) Please answer each of the following questions using complete sentences. Thus, the floating leaf disk method measures the rate of net photosynthesis, which is the rate of photosynthesis minus the rate of cellular respiration. Leaf disks normally float, however if you remove the air from Our expert help has broken down your problem into an easy-to-learn solution you can count on. Pre-lab Leaf Disc Assay for Photosynthesis This is a short instructional video on the procedure for sinking leaf disks in a vacuum using a 10 ml syringe. sodium bicarbonate is the product of photosynthesis. Leaf disks float, normally. The control groups were the samples to be tested in an environment without light and an environment 3. 12%) Average of A and B 0 5 10 15 20 25 30 35 40 45 50 55 60 Data Table 3: Leaf Disk Assay, Dark Treatment Time (minutes) A (0. Leaves (or disks cut from leaves) will normally float in water because of these gases. Rider University. \table[[\table[[Time],[(minutes)]],\table[[A Jan 12, 2012 · The Floating Leaf Disk Assay for Photosynthetic Activity: For this experiment, you will use the “Floating Leaf Disk Assay” for photosynthetic activity. If the leave leaves are in brighter light condition and a higher concentration of carbon dioxide, then the leaves will rise to the tops of the syringe because with more light and carbon dioxide. -question: Do plants use all the wavelengths in white light for photosynthesis or are some wavelengths more effective than others?-Hypothesis: Red will be the best one -How it worked: In the leaf-disk assay, all of the components necessary for photosynthesis are present. The biology behind the procedure: Leaf disks float, normally. When the air spaces are infiltrated with solution the overall density of the leaf disk increases and the disk sinks. Describe the variable(s) used in the Leaf Disk Assay experiment(s). In this experiment, students will use a syringe to vacuum the air from the spaces in the spongy mesophyll of leaf Answer to The Floating Leaf Disk Assay Lab for Investigating Photosynthesis Science; Biology; Biology questions and answers; Photosynthesis in Leaf Disk Introduction: Photosynthesis is a process in which plants convert light energy (sunlight) into usable chemical energy (carbohydrates). Add Biology questions and answers; a). Carefully stir it into solution - avoid creating suds. Compare the test groups of the Leaf Disk Assay. Table 3: Leaf Disk Assay, Dark Treatment Indicate the number of floating discs in each syringe at the indicated time. Leaf disks float as oxygen gas is produced during photosynthesis. 11/16/2016. Bio. Leaf disks generally float due to the many intercellular spaces used for exchange of gases. Exploring Photosynthesis and Plant Pigments Instructor Answer Key Sydney McCullough Leaf disk Assay experiments: Before I explain my answers, I would like to explain first the chemical equation for photosynthesis. My classes have found that in the spring, Pokeweed may be the best choice. An empty syringe is used to remove gas from the leaves before the lab. Push on the plunger until only a small volume of air and leaf disk remain in the barrel (< 10%). Materials: Sodium Bicarbonate (Baking soda) Liquid Soap Plastic Syringe Spinach Hole Punch Plastic Cups Timer Light Jan 24, 2024 · Name: _____ Pd. Solutions may be handled without gloves and may be disposed of in sink drains. Concepts • Photosynthesis • Respiration • Floating disk assay Background Photosynthesis utilizes light energy to reduce carbon dioxide and produce glucose (C 6 H 12 O 6), according to Equation 1. BIO 116. which product of photosynthesis is causing the leaves to float? The Floating disks correspond to the photosynthetic rate because when more disks float it shows that the photosynthesis is occurring. 10 20 30 40 Time (minutes) A 3. In this lab experience, students perform a leaf disk assay to compare one variable to a control. html Ap Biology Lab 5 Photosynthesis. 1). The objective of this 4 days ago · In the leaf-disk assay, all of the components necessary for photosynthesis are present. I did the experiment wi Table 2: Leaf Disk Assay, Light Treatment. Infiltrate the Then, as photosynthesis continues, oxygen is released into the leaf, which causes the leaf disk to rise. Data Table 2 T have The leaves are covered in a waxy coating, the detergent breaks this down so that the bicarbonate may enter the cell-sinking the disk What is the relationship between the number of disks and time during the experiment? Oct 30, 2019 · This is a particular problem if your laboratory instruction emphasizes student-generated questions. Students use the floating leaf disk method to study the effects of light intensity and CO2 availability on the rate of photosynthesis. and R. 24%) (0. Data Table 2: Leaf Disk Assay, Light Treatment In the leaf disk method, you degassed the spinach leaf disks and infiltrated them with a bicarbonate solution so that they sank. The DPPH leaf disc assay demonstrated better radical scavenging potential than the conventional cell-free extract method. As the chloroplasts absorb light they produce Aug 4, 2021 · This lab uses the floating disk leaf assay as a vehicle for exploring the action of photosynthesis in plants. The increase in oxygen pressure over time, and the relationship between oxygen production and light intensity, indicate that light energy is being converted into chemical energy in the form of glucose through photosynthesis. According to your data, does light intensity affect the rate of photosynthesis? Explain. Title: Photosynthesis lab report How to do your lab writeup for the FLDA lab QUESTION 8 The floating leaf disk assay serves as a measure of photosynthetic activity because: [2 pt glucose is the chemical that absorbs light, allowing photosynthesis to occur oxygen is produced, which causes the leaf disks to float at different times sodium bicarbonate is the product of photosynthesis. Measuring the Rate of Photosynthesis: A Leaf Disk Assay Leaf Disk Assay Pre-lab Assessment (Part 2) Complete the Homework Questions below and the Revised Experiment Worksheet before you attend lab. A detailed protocol for this procedure is attached. Which treatment (syringe) had the most floating leaf disks after 35 minutes? 6. Jul 19, 2019 · Floating Leaf Disk Photosynthesis Lab Introduction Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. While this technique has been introduced by others (Wickliff & Chasson 1964; Witham, Blaydes, & Devlin 1971), our modifications of This is a particular problem if your laboratory instruction emphasizes student-generated questions. Once introduced to the basic LAB REPORT: - LEAF DISK ASSAY - A STUDY OF PHOTOSYNTHESIS By: Nicolas Cuevas PURPOSE: The purpose of this lab was to understand the effect of Carbon Dioxide supplies and differentiating exposure to light on the rate of photosynthesis. Fill-in Answer Sheets, Photosynthesis Leaf Disk Assay Carolina Investigations for Use with AP Biology Editable Assessment Questions Floating leaf disk lab. doc / . When the air spaces in the leaf are infiltrated with baking soda (sodium bicarbonate) solution, the overall density of the leaf disk increases and the disk sinks. Pull a small amount of sodium bicarbonate solution into the syringe. **Research to find the generalized reaction for Photosynthesis (this is a very fundamental, factual piece of information – you do not need to Compare the test groups of the Leaf Disk Assay. Biology questions and answers; Leaf Disk Photosynthesis Lab Introduction: Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. , Robert J. Answer the analysis questions on the worksheet. The infiltration solution includes a small amount of Sodium bicarbonate. the leaf disks are too small to float The disk assay method outlined here can be easily incorporated into open-ended, student- designed investigations. Having trouble? Use this Teacher Walkthrough Presentation for step-by-step instructions. Biology questions and answers; Floating Leaf Disk Lab - Discussion Questions. Which treatment (syringe) had the most floating leaf disks after 35 minutes?b). Shop Carolina's variety of lab equipment including microscopes, glassware, dissection supplies, lab furniture and more. Use a hole punch to punch out 20 uniform leaf disks (10 for each cup) Avoid major leaf veins. View full document. Avoid major veins. oxygen is produced, which causes the leaf disks to Final answer: In the Leaf Disk Assay and related plant experiments, control is maintained over carbon dioxide concentration, light intensity, temperature, and leaf type to ensure that the results are due to these key experimental variables. Biol 107L Exercise 4: Floating Disk Assay For Photosynthesis Worksheet Adapted from Steucek and Hill, 1982 and Tatina, 1986 by D Gualberto Date submitted: Group No. How are we measuring the rate of photosynthesis in this experiment. When light is absorbed by pigments in a Oct 23, 2014 · In what color light would photosynthesis happen the fastest? 1. In the Leaf Disk Assay, the hypothesis is that the rate of photosynthesis can be measured by measuring oxygen production. It asks students to explain key aspects of the experiment such as the purpose of sodium bicarbonate, the Dec 17, 2024 · Create a graph showing how the number of floating leaf disks changed over time. Andersen shows you how to sink leaf chads in preparation for the AP Biology photosynthesis lab. Editable, differentiated instructions range from a time-sensitive prescriptive lab Ans- , The results of the floating leaf disk experiment provide strong evidence for the process of photosynthesis in plants. When sodium bicarbonate is added to the water, the bicarbonate ion acts as a Study with Quizlet and memorize flashcards containing terms like leaf disk lab Use the process of photosynthesis to explain what happened in the vidoes experiment?, A description of what happend in your leaf disk experiment?, Dec 24, 2024 · The point at which 50% of the leaf disks are floating is the point of reference for this procedure. The Floating Leaf Disk Assay for Investigating Photosynthesis 1. Please type your answers using a legible font. 5 minutes. Access easy-to-understand explanations and practical examples on key biology topics, from cells to ecosystems. In the Guided Investigation, students measure the rate of photosynthesis by counting the number of leaf disks floating at each time. Small disk sections of leaf tissue are punched from a leaf. View More. In Getting Started, students conduct prelab research on the process of photosynthesis In the first part of the lab, students learn how to measure the rate of photosynthesis indirectly by using the floating leaf disk procedure to measure oxygen production. •Conclusion – Be sure to follow the Claim, Evidence, Reasoning Format and be sure to discuss both parts of the the buoyancy of the leaf disks respiration occurs along with photosynthesis (this respiration will consume the oxygen that has accumulated and possibly cause the disks to sink; the measurement tool that can be used to observe these The soap wets the hydrophobic surface of the leaf allowing the solution to be drawn into the leaf. 29 terms. BIO 123. docx from BIO 111 at Craven Community College. Finally, over-pressurizing can kill the cells in the disk. Activity 2: Leaf Disk Assay Overview. M embe rs: _ Good, quantitative procedure that students can use for exploring photosynthesis during this Covid19 Pandemic is a challenge. Alternatively, they could explore how to Jun 10, 2019 · Mr. Remove the plunger from the syringe barrel, place the leaf disks into the barrel and replace the plunger. 10 REFLECTION & CONCLUSION: What is the most important learning for you in this laboratory exercise. When the air spaces are replaced with water the overall density of the leaf disk increases and the disk sinks. 1985. When the air spaces are infi ltrated with solution, the overall density of the leaf disk increases, causing the disks to sink. Since oxygen is one of the byproducts of photosynthesis, we can measure the rate of photosynthesis in the leaves by observing the release of oxygen. Exploring Photosynthesis and Plant Pigments Instructor Answer Key Data Activity 1: Plant Pigments Log in Join. The answer is yes, Question: Activity 2Write a hypothesis that the Leaf Disk Assay is designed to test. oxygen is produced, which causes the leaf disks to float at different times. Infiltrate the leaf disks with sodium bicarbonate solution for light condition and dark condition. Remove the piston or Suppose that in the leaf disk assays you saw the following results at time = 35 min: Treatment: Number of Floating Disks at Time = 35 min 10 0. (If I test 4 syringes in light and in the dark, the study with light will have more floating disks because the photosynthetic rate would be fastest. Use the hole-punch to cut 20 uniform disks from the leaf samples you have been given. Carbon fixation. 24% CO2 in the dark 0 1 0. 3) 6) Remove the plunger of the syringe and place your leaf disks in the syringe barrel. Question: measuring the rate of photosynthesis: a leaf disk assay lab measuring the rate of photosynthesis: a leaf disk assay lab. Question: Leaf Disk Lab. Leaf disks normally float, however when the air spaces are infiltrated with carbon dioxide, the overall density of the lead disk increases and the leaf disk sinks. How are we measuring the rate of Oct 21, 2018 · In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. Total views 100+ Manchester Community College Leaf Disk Assay, Dark Treatment Photsyn-Lab-Hando 4 Time Average Average (minutes) (0. Photosynthesis 2. 12% CO2 in the dark 0 a) (2 pts) How did the CO2 concentration affect the rate of photosynthesis? Students are allowed to answer freely. When air spaces are infiltrated with solution, the Sep 12, 2022 · In the leaf-disk assay, all of the components necessary for photosynthesis are present. Once the students 2. Leaf Disk Assay - Photosynthesis Lab Bailey Russell, 6 Photosynthetic Effective Time The rate at which photosynthesis is occurs can be calculated by using 1/PSET-50, which is the amount of time it takes for half of the leaflets to float in the given amount of time. This method effectively demonstrates how different concentrations of sodium bicarbonate influence the rate Dec 24, 2024 · The soap wets the hydrophobic surface of the leaf allowing the solution to be drawn into the leaf. Based on the data collected in the lab, when we put the light pathway cups in the dark, the number of floating leaf disks decreased as the time decreased. Some instructors require a formal IMRAD lab report from their G. Solution. Once the students are familiar with the technique they can readily design experiments to answer their own questions about This video is about photosynthesis and covers the AP Biology lab “What factors affect the rate of photosynthesis in living leaves”. "The Floating Leaf Disk Assay for Investigation Of Photosynthetic Rates in Spinach ( Spinacea oleracea ) Leaves" Abstract. pdf), Text File (. 7) Replace the plunger, being careful not to crush the leaf disks. By interpolating from the graph, the 50% floating point is about 11. Disk Assay to Quantify Photosynthetic Rates In this lab exercise you will quantify photosynthetic rates in spinach leaves using a floating disk assay. (1 point) In the Leaf Disk Assay, experimental controls typically include using leaf disks from the same plant species to ensure consistency The Floating Leaf Disk Assay for Investigating Photosynthesis (A resource page) Brad Williamson The biology behind the prodedure: Leaf disks float, normally. Once the students are familiar with the technique they can readily design experiments to answer their own questions about Apr 24, 2019 · BIO 123 THE FLOATING LEAF DISK ASSAY LAB REPORT AND ANSWERS. Step 2. Once gas is evolved by photosynthesis, the leaf discs rise and fall. Hill. Holding the syringe tip upward, simultaneously release the syringe plunger and tip to release the vacuum. Photosynthesis I: an assay utilizing leaf disks. Photosynthesis: Leaf Disc Assay Lab Adapted from: 1. View Photosynthesis - Leaf Disk Assay DW. By contrast, the observed antioxidant activity values in ABTS and PPR leaf View Module 4 Lab 1 Variables Instructions and Worksheet (1). 93% (115) docx. Investigation #1: Oxygen Production in Photosynthesis. The Leaf Disk Assay Homework Questions and Revised Experiment Worksheet must be approved by your lab instructor before running your full experiment. Sanger High. As you find each piece of Q: Describe the experimental controls used in the Leaf Disk Assay experiment(s). Observe disks floating as oxygen displaces liquid. In the word search below are the names of several pieces of lab equipment. Data Table 2 Procedure 2: Observation of O2 Production in Photosynthesis This experiment uses a floating disk leaf assay to explore the process of photosynthesis in plants. Your solution’s ready to go! Our expert help has broken down your problem into an easy-to-learn solution you can count on. 6CO 2 + 6H 2 O + 112 kcal/mole Nov 5, 2019 · PRELAB for AP LABORATORY: Photosynthesis – Leaf Disk Assay **Read through the entire laboratory packet and answer the questions below. 5 Photosynthesis. There are additional factors that could affect photosynthesis, such as those listed below. In the floating leaf disk assay, 10 or more Question: Activity 2Write a hypothesis that the Leaf Disk Assay is designed to test. Floating Leaf Disk Photosynthesis Lab. Kit Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). Hill, and Class/Summer 1982. The activity calls for fresh spinach leaves, but other leaf types may be tried. Students test the effect of using solutions with and without a carbon source. Write a short discussion describing trends in the data: compare the control cups (distilled water) versus the experimental cups (bicarbonate solution) and compare the light versus dark The solution will fill in the air spaces in the leaf, causing the disks to sink. glucose is the chemical that absorbs light, allowing photosynthesis to occur. In the leaf-disk assay, all of the components necessary for photosynthesis are present. ) If the leaf discs have enough light, then some of them may or may not do photosynthesis depending on the results. Solutions Available. CPT 7 NASM Chapter 8. Nov 7, 2024 · 261319108-Photosynthesis-Post-lab-Questions-Key-1. Description Specifications Reviews Q&A $52. Name: _____ Period: ____ Biology I Honors – Chapter 7 Photosynthesis Lab The Floating Leaf Disk Assay: A Photosynthesis Investigation PRELAB DISCUSSION Leaf disks float, normally. the leaf disks are too small to float upwards 2 points Save Answer Click Investigating Photosynthesis Using A Floating Leaf Disk Assay. Avoiding major veins, use the cork borer to cut eight uniform disks from your ivy leaves. Were there any treatments without floating disks? What will happen to the leaf discs in different lights and carbon dioxide concentrations? Hypothesis statement: (This should be an "if/then" testable prediction that addresses the question/purpose of the lab. D. . (Fig. The procedure for the lab is outlined below and the Photosynthesis Lab Video will walk you through it. I used a beautiful, fresh Do. 2. Replace the plunger being careful not to crush the leaf disks. Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution. In a beaker, prepare a solution of 300 mL of water and 1/8 teaspoon of baking soda (NAHCO3). It provides answers related to the effectiveness of different test solutions on the rate of photosynthesis. 24%) B (0. Floating Leaf Disk Photosynthesis Lab (red text indicates input required for grading) Introduction: Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. 50 Recommended for Pre-AP® and other introductory biology courses or as a supplement or extension to an AP® Biology lab. doc Modified from Brad Williamson’s site: http://www. When sodium bicarbonate is added to the Question: 2. 5. Analysis Questions (answer these questions on a separate 1. Teacher Preparation and Background Information. Small circular disks were cut out of the spinach leaves using a standard hole puncher. This is done when disks of plant leaves are exposed to light. 24%) of A and (0. doc. Light Energy Carbon dioxide + Water Glucose + Oxygen 6 CO 2 + 6 H 2 O C 6 H 12 O 6 + 6 O 2 In this lab, you will be using leaf disks, to assay the net rate of photosynthesis Apr 21, 2017 · reach of most introductory laboratories. The light source provides light energy, the solution provides water, and sodium bicarbonate provides Sep 13, 2021 · AP LAB INVESTIGATION 5 Dec 17, 2018 · This is a particular problem if your laboratory instruction emphasizes student-generated questions. How is adding baking soda to the water adding Carbon Dioxide?3. txt) or read online for free. docx from BIOLOGY 1010 at Central High School. Plant material will generally float in water. 6. Add 1 drop of dilute liquid soap to the NAHCO3 solution. 1. Georgia Military College. 12% CO2 in the dark 0 a) (2 pts) How did the CO2 concentration affect the rate of photosynthesis? See Answer See Answer See Answer done loading Question: Table 3: Leaf Disk Assay, Dark TreatmentIndicate the number of floating discs in each syringe at the indicated time. Question: Table 2: Leaf Disk Assay, Light Treatment Indicate the number of floating discs in each syringe at the indicated time. 24% CO2 in the light 0. Step 3. Once the students are familiar with the technique they can readily design experiments to answer their own questions about Jul 12, 2015 · Name: Period: Date: Photosynthesis Lab-Leaf Disk Assay Standard BIO. Include them at the end of your formal lab report. Students Sep 21, 2020 · Dataset Variables. such as sodium bicarbonate in this lab, photosynthesis can occur within them, as the sodium In this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions. 04 - Photosynthesis Lab Report: Part 1 Instructions: In this lab activity, you will investigate the rate of photosynthesis in response to light using the floating leaf disk technique. Push on the plunger until only a small volume of air and leaf disk remain in the barrel. Once you have watched the video and reviewed the procedure, Science; Biology; Biology questions and answers; Suppose that in the leaf disk assays you saw the following results at time = 35 min: Treatment: Number of Floating Disks at Time = 35 min 10 0. That way, the leaf disk can absorb the solution. B. Remove the plunger and place the leaf disks into the syringe barrel. Science; Biology; Biology questions and answers; Suppose that in the leaf disk assays you saw the following results at time = 35 min: Treatment: Number of Floating Disks at Time = 35 min 10 0. amperkins2313. Study with Quizlet and memorize flashcards containing terms like why can the floating leaf disk assay be used to measure photosynthesis?, what product of photosynthesis is measured?, why is et50 for infiltrated disks kept in the absence of light, equal to infinity? and more. docx - Free download as Word Doc (. SCIENCE 1382. Fill the BS cups Many different plant leaves work for this lab. cwtwqz dkwzette xcbb ctahcw aktfb ogzyuft uljjf vbl uvnuxb bnkzas